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Chronic Spontaneous Urticaria (CSU)

Work-Up

History, physical, basic tests, assess disease activity/impact/control, labs [1] - 7Cs:

confirm (rule out other disorders), cause (look for markers of autoimmunity), co-factors

(identify triggers), consequences (other autoimmune disorders, QOL), components (predictors of treatment/biomarkers), course (monitoring CSU control) [3]


Recommended laboratory work-up

- CBC w/ diff (look for eosinophilia, which may indicate underlying disease; may see

basopenia and eosinopenia in 10-15% CSU [2, 3])

o Baso-/eosinopenia, low IgE may predict poor response to H1 anti-histamines and/or

omalizumab [3]

o May be inaccurate if patient recently received systemic glucocorticoids - anti-TPO

Ab: anti-TPO IgG more commonly measured vs anti-TPO IgE [3] o Serologic testing for anti-TSH receptor and anti-thyroglobulin antibodies can be considered, although data on their association with CSU is less robust than for anti TPO

o Intact omalizumab responsiveness in patients with anti-TPO IgE antibodies in one

study (dosing was based on total IgE) [11]

- Total IgE: conflicting evidence - some studies show no difference in total IgE in autoimmune vs non-autoimmune CSU [2], but other studies have demonstrated low total IgE in autoimmune CSU [13]

o High levels may indicate alternative etiology

- Obtain TSH, free T4 if history supports thyroid dysfunction and/or if anti-thyroid antibodies present

- CU index: in vitro basophil histamine release assay; indicates anti-IgE/-FcεR antibodies (RI

or II) or presence of histamine-releasing serum factor) [18]

o May indicate more difficult-to-treat disease – some studies have demonstrated higher values in refractory CSU cases (symptomatic despite anti-histamines +/- leukotriene receptor antagonist) [19, 20]

- CRP, ESR

- C4 if isolated angioedema

- Some guidelines recommend CRP and ESR in all, with anti-TPO IgG and total IgE for those receiving specialist care [1, 3]

- 80-90% of patients have no identifiable cause [21]


Secondary work-up to consider (based on history, exam, other labs [1])

- H. pylori stool antigen test- Toxacara canis antibody test

- Stool ova and parasites

- GI referral (if concern for inflammatory bowel disease)

- ANA, other rheumatologic labs

- IgE antibody (anti-IgE IgG), CU index

- Tryptase if suspected mastocytosis and/or hereditary alpha-tryptasemia (HaT) o May be

elevated in CSU, including into abnormal range, and may correlate with disease

activity [22, 23]

- Specialist care: consider based on history, exam, other labs [1]

o Food, drug or other trigger testing

o Paraproteinemia

o Gene mutation analysis for hereditary periodic fever syndromes (especially if

pediatric patient and episodic symptoms; rash, ulcers, lymphadenopathy,

abdominal pain, pharyngitis, conjunctivitis)

o C4, C1-INH level and function, C1q, C1-INH antibodies if suspecting bradykinin

mediated angioedema

- Autologous serum skin testing: plasma or serum obtained during a period of active

disease injected intradermally (IDT; 0.05 mL) in area of uninvolved skin; wheal ≥ 1.5

mm larger than saline control is suggestive of autoimmune etiology [2]

- IL-6 may correlate with CRP and disease activity, although is not regularly utilized in

clinical practice [24]

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